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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in several tumors, which leads to chromosome instability, and therefore might contribute to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial job in selling mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants were being described but comprehensive nucleotide sequence information and facts is just not however out there.
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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide usually includes the epitope identified by the antibody.
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This guide outlines additional possible optimization of mobile and immunoassay parameters to guarantee the absolute best success are...
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This tutorial outlines further more possible optimization of mobile and immunoassay parameters to ensure the best possible results are...
Antibodies certain to the blocking peptide not bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs on the really conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation styles which contribute to your temporal coordination of each mitotic occasion. Cyclin E varieties a complex with and capabilities as being a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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